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Daily news and analysis about the food tech revolution

CRISPR

Four Predictions for the Future of Food in 2021

It goes without saying that 2020 was a challenging year for the food industry. A worldwide pandemic that wreaked havoc on food supply chains, forced the permanent closure of thousands of restaurants worldwide, and pushed millions of people deeper into food insecurity showed us just how fragile the systems that keep us nourished and fed are.

But it’s also the recognition of this fragility that’s led to an increasing sense of urgency to invest in the future of food. The good news is the timing couldn’t be better. We are at a culmination point in the fields of bioengineering, chemistry and food science where decades of hard work and progress have allowed ideas that once seemed the domain of science fiction to leap into the labs and, now and in the not-to-distant future, onto our plates.

And while 2020 was a year of unprecedented progress across our food system, I expect 2021 to be even more impactful. Below are four predictions for some of what we could see this year.

Cultured Meat Milestones Will Accelerate

Throughout 2020, announcements of milestones for cultured meat flowed with increasing regularity. New prototypes of practically every type of meat ranging from chicken to beef to kangaroo debuted, heads of state and other famous folks got their first tastes of lab-grown meat, and at the end Eat Just announced the first regulatory approval and retail sale of cultured chicken in Singapore.

And we’ll see even more milestones this year. Investment will grow and excitement will build as more companies move out of the labs and into early pilot production facilities for their cultured meat products. Other countries will follow Singapore’s lead and give regulatory green light for the sale of cultured meat. And finally, we’ll see the debut of more cultured meat products in high-end cuisine as chefs look to achieve similar firsts for their restaurants. We may even see the rollout of cultured meat in some select experiential, high-end retail.

Fermentation Powers Growth in Exciting New Consumer-Facing Products

One of the of most exciting areas in the future of food is microbial fermentation. High-volume production of interesting new biomass proteins such as mycelium-based meat replacements and the arrival of animal-free proteins, fats and other compounds created using precision fermentation helped illustrate why the Good Food Institute called fermentation the third leg of the alternative protein market.

Looking forward, you can expect lots of new products to debut powered by precision fermentation in 2021. MeliBio, a maker of bee-free honey, expects to debut their first product in 2021, while Clara Foods plans to release its animal-free egg this year as well, and I expect to see more companies like Brave Robot rise up and offer new products built around precision fermented food platforms created by companies like Perfect Day.

CRISPR and Gene-Edited Food See Accelerated Product Pipelines

There was big news in the CRISPR and gene-edited food realm in December when the USDA proposed a change in the regulatory oversight of gene-edited animals for human consumption. The organization proposed that they take over oversight responsibility for approving gene-edited animal products from the FDA which, in 2018, famously declared that gene-edited animals should be regulated in the same manner as drugs.

Under a new USDA regulatory framework, the organization is proposing a fairly light regulatory approach to animals compared to the previous oversight of the FDA, which in turn could speed up time to market for new products. While there has been lots of focus on CRISPR-derived future food innovation, I expect changes to US regulatory oversight of gene-edited animal products to create a wave of new interest in developing CRISPR-based product lines from both startups and established food product companies.

Finally, the US may not be the only market to see a change in oversight for gene-edited food. The UK is looking to extract itself from the heavier-handed oversight of the EU post-Brexit, and some in Europe are suggesting that the EU’s classification of all gene-edited food as GMO might be overbroad and need adjusting.

3D Food Printing Moves Beyond the Cake

While 3D food printing has largely been relegated to the world of confections and cake decorating, a world with food replicators from the pages of science fiction novels seems to be inching closer to reality.

Companies like Redefine Meat are making high-volume plant-based meat printers and plan to have meat in supermarkets in a year, while others like Meat-Tech are showing off prototypes of cultured meat printers. One of the challenges for food printing will be scaling the technology to make it quicker, something Novameat is working on as it begins to enter commercial rollout phase of its plant-based meat printing technology. On the consumer front, while I don’t expect the food printers to start printing out Jamie Oliver recipes this year, companies like Savoreat are working on commercializing products for the professional space with the end-goal of eventually creating a home consumer food printer like the one you might see in a show like Upload.

Finally, these advances and technologies do not happen in a vacuum. The future of food is reliant on a multitude of new innovations and technologies. CRISPR, precision fermentation and 3D food printing are just some of the tools being interwoven and utilized together to help bring innovative new products to cultured, plant-based and other emerging food markets.

While we don’t know what 2021 will hold for us with any certainty, what we can be certain of is that progress in these important building blocks for the future of food will continue to march forward.

CRISPR’d Cows: Proposed Rule Change By USDA Could Accelerate Gene-Edited Animal Production

CRISPR had quite a 2020. Not only did the cutting edge genetic engineering technique give us hope for better and faster COVID-19 tests and help advance new treatments for diseases like cancer, but its creators also received the world’s most prestigious award in science when they received the Nobel prize for chemistry for their pioneering work.

CRISPR’s also been marching forward in the food world. Pairwise, for example, made regulatory headway this year in advancing gene-edited produce in the form of a mustard green that actually tastes good and has a strong nutrition profile.

However, while we’ve seen some limited momentum when it comes to animals and CRISPR, such as making chickens more resistant to avian leukosis virus, regulatory approval for gene-edited animals has been slower ever since the FDA declared that molecularly manipulated animals needed to be regulated like drugs.

But that may soon change. Last week, the U.S. Department of Agriculture (USDA) pushed out a proposed rule change suggesting that they take over the regulatory oversight of gene-edited animals from the FDA.

According to the proposed rule change, regulatory oversight of gene-edited animals for human consumption would be transitioned almost entirely to the USDA. From the release:

USDA would in most instances serve as a single point of entry for amenable species modified or developed using genetic engineering that are intended for agricultural purposes and would provide coordinated end-to-end regulatory oversight from pre-market animal pest and disease risk and human food safety reviews through post-market human food safety reviews for amenable species modified or developed using genetic engineering intended for use as human food.

First flagged by Wired, the news is an important potential development as the USDA – which has had oversight of CRISPR regulation for plant-based food – has taken a fairly laissez-faire approach relative to other US agencies. In 2018, the agency declared that CRISPR-edited crops would not require additional regulation.

With the proposed rule change, the USDA now wants to take an accelerated approach when it comes to gene-edited animals, a change that would also expand the scope to include oversight on whether the bio-engineered animals are safe for human consumption.

According to the proposed rule change, if the genetically modified animal was intended for human food consumption, the animal would undergo a risk-based and science-based review focused on food safety (in addition to animal health).

During this review, the proposed rules state that if the “USDA finds that the modification made using genetic engineering is equivalent to what can be accomplished through conventional breeding practices, the animal would not be subject to further regulation under the contemplated regulatory framework.”

If there were are resulting changes to the molecular structure of the animal outside of what would happen during traditional animal breeding – such as unintended DNA insertions – the agency would then notify the party responsible and a permit would be required to import, sell or release such an animal into a wider population.

The proposed rule change would cover pretty much all the same animal species the USDA currently regulates, from cattle and sheep to fish and poultry. The notice of proposed rule change is currently in the public comment period, which will last through February 26, 2021.

While the U.S. was the early leader in CRISPR-based intellectual property, China’s actually taken the lead when it comes to CRISPR-based agriculture innovation. This move by the U.S.’s primary food and agriculture regulatory agency to relax its oversight could help the country regain momentum as the world’s two largest economies continue to battle it out in this important future food battleground.

Mosa Meat's steak tartare on white plate with garnishes

Cultured meat takes sides on CRISPR

In 2017, a patent assigned to Memphis Meats detailed a way to overcome one of cultured meats biggest obstacles. The startup would use CRISPR gene editing to create a small mutation in their cells. The mutation would inactivate two proteins and ultimately increase “replicative capacity of the modified cell populations indefinitely.” They had transformed unpredictable cells with a limited capacity into hyper-proliferative ones  equipped for industrial production.

Longevity and predictability are the obstacles all cultured meat start-ups face in the effort to bring production to scale. Commercial scale cultured meat will require a mass production of cells like no other project to date, but cells in question aren’t inherently capable of that kind of output. After a certain number of replications, the fat, muscle and connective tissue cells max out. They  begin to die off or lose control. Left to itself, cultured meat eventually becomes self-contaminating. 

CRISPR gene editing offers a work around, a cheap and accurate way to equip stem cells for industrial capacity and consistency. Muscle and fat stem cells that naturally peter out can be edited to divide forever. Induced pluripotent stem cells that easily veer off course can be reprogrammed to exclusively produce muscle cells, fat or connective tissue. 

“Technologies like CRISPR allow us to safely increase the quality of our cell growth, which means we will make meat that is tastier, healthier, and more sustainable than slaughtered meat,” Brian Spears, the co-founder and CEO of New Age Meats, told Business Insider last year. Ostensibly, genetic tweaks made using CRISPR could make industrial cell culture faster to market, more predictable, and more cost effective.

But while some start-ups make CRISPR gene editing intrinsic to their process, others are intentionally separating themselves from the technology. They’re concerned that genetically altering their cell lines could lead to regulatory hang-ups — if not in the US or Asia, then in Europe. They’re calling their cultured meat non-GMO.

Whether CRISPR is a GMO has been hotly debated since the technology was first adapted for research from bacterial defense systems. Unlike genetically modified organisms, which have had foreign genetic material inserted into their DNA and been edited in a way that couldn’t occur naturally, CRISPR alters an organism’s own DNA to exhibit the most desirable traits. 

“Scientifically I buy that it’s not a GMO,” Paul Mozdziak, a cell biologist at North Carolina State told me via Zoom, “but regulation is often based on more than science.” Mozdziak is also an affiliate of Peace of Meat, a B2B cultured meat company that’s decided against CRISPR. “Our profile is we are not going to do anything that can be construed in any way shape or form as GMO,” Mozdziak said.  The same is true of Mosa Meat, a cultured meat elite who produced the first lab-grown burger in 2013. The decision is partly because Mosa is in the European market which doesn’t have a favorable attitude toward CRISPR at all, said Joshua Flack, cell biologist and leader of Mosa Meat’s Stemness & Isolation team. But “It also makes scientific sense. It is a lot of work to engineer your cell lines in this fashion.There’s a lot of ground work in the beginning if you’re using CRISPR and engineering.”

For those that don’t go the CRISPR route, the key is identifying the optimal cell line, finding out exactly what those cells want, and then catering the entire process to them, Flack said. The non-GMO approach is about optimizing the process while CRISPR offers a way to “turn the thing on its head” by genetically optimizing the cell line.  

From a scientific standpoint, no one is challenging CRISPR’s potential. Mozdziak called it a “promising technology” for the entire industry and even expects US regulatory bodies to be fairly amenable to the technology. Meanwhile, Mosa Meat has invested in inhouse explorations using CRISPR for R&D purposes. “We have to understand the risks of not employing these strategies,” Flack said. “The potential upside is really massive.”

CRISPR could very well be the fastest and cheapest way to commercial scale, but it’s unclear how much that will matter in the long run. Which process will be first to market or which will be stalled in regulations? These questions are just proxies for the one question that we can’t answer yet. That is, what will people buy–and buy enough to disrupt the meat industry? Maybe this new age GMO debate ends like the last one: both sides proceed so customers have the option. But one thing is for sure, Flack said,  “if you can’t sell it at the end, the effort is wasted.”

UPDATE: An earlier version of this article contained a quote from Daan Luining, CTO of Meatable. For administrative reasons, that quote has been replaced with a quote from Brian Spears, CEO of New Age Meats, originally published by Business Insider.

Pairwise Gets Greenlight from USDA for CRISPR-Engineered Mustard Greens

While health benefits of mustard greens have long been known, the strong bitter taste and pungent smell has kept this leafy green from going as mainstream as lettuce or kale.

But that could change soon. That’s because CRISPR-focused produce startup Pairwise got approval in mid-August from the USDA for their gene-edited version of Brassica juncea, more commonly known as mustard greens.

According to a release sent to The Spoon, the USDA sent confirmation to Pairwise in mid-August that the company was approved to move forward with their new take on a leafy green that has not been on many menu due to a pungent smell and bitter taste. With changes engineered by CRISPR technologies, Pairwise hopes to create a nutritious alternative to kale and Brussels sprouts that also tastes good.

Pairwise Greens in the field

According to Pairwise, their new mustard greens will feature a strong nutrition profile with supple leaves that hold up well to salad dressings and toppings and will have green and deep purple colors.

When I interviewed Pairwise CEO Tom Adams a month ago, he told me one of the reasons the company focused on mustard greens was the high yield of the crop.

Mustard greens “look just like lettuce, they they feel like lettuce, so if we could eliminate that flavor and make them taste like lettuce that they that they would be like lettuce,” said Adams. “It turns out they actually have another attribute, which is they yield on an acre basis. They yield about three times as much as kale.”

According to Pairwise, they currently have five varieties of the gene-edited produce in field trials. They have plans to expand the field trials in a few months to include the edited product and will provide sample products to partners this fall.

How CRISPR Could Create Produce That Lasts Longer, Tastes Better, and Won’t Make Pickers Bleed

Pairwise is one of the companies making a name for itself developing new types of products using CRISPR. The company is developing consumer-facing brands of produce that offer unique characteristics created through the use of CRISPR toolsets.

I caught up the CEO of Pairwise, Tom Adams, to discuss what the company is working on and to get his thoughts on how CRISPR will change the food system. Below are some excerpts from my interview. Spoon Plus subscribers can watch the interview and read the full, unedited transcript.

What are some examples of these types of CRISPR products with direct consumer benefits?

So a product that we’re interested in, sort of it’s a longer term product, is to create a cherry without a pit. You can imagine being able to just pop a cherry in your mouth and really enjoy that healthy, healthy fruit. Cherries are in season right now. They’re great, but I keep ending up with purple fingers from eating them all. I’d love to be able to just pop them over my mouth and eat them like grape. So that’s the kind of thing where we’re taking it down the barrier so that a consumer can really enjoy the cherry differently.

Now we’ll do other things that help with the overall production system. One of our ideas with cherries is to make it so you can produce cherries year round like we’ve done through 60 years of breeding with blueberries. We now have blueberries every day and I didn’t use to get blueberries every day but now I do.

How could CRISPR could accelerate the development of new forms of produce compared with traditional cross-breeding of crops?

There actually is a pitless plum that somebody isolated a few years ago. It’s not a good tasting plum, so it’s not a variety that sold. But you can cross plums and cherries, and you get chums or clerries or something. It’s not a cherry or a plum anymore. The Bing cherry was bred in 1880 and Bing cherry since then is a clone of that original tree. So if you cross them, that’s not a Bing cherry anymore.

You want to get back to the Bing cherry, you’d cross the chum back to the cherry probably 7 or 8 or 9 times until you get a little bit more cherry genome in it each time until you’re almost cherry again. That’s probably 150 years from now you’d have a pitless cherry. But with gene editing, I know what the mutation is that really resulted in the loss of the pits, so I can just go directly into the cherry and make that mutation. It’s the same endpoint that I would have gotten to through the breeding. It’s just 150 years faster.

One thing you’re working on is berries. Can you tell us more?

The blackberries I buy in the grocery store, I could take or leave. And that’s because they’re the variety that had some mutations in it that allow it to be more productive through the season. This mutation just happens to be in a variety that just doesn’t taste good. It’s very high acid. It’s not a really great berry. So we’re taking berries that taste like the ones in the Northwest and we’re putting in the same mutations that you’d see on the bad tasting ones that allow for higher productivity, and adding those to the really good tasting one. And then, just for good measure, we’re also going to get rid of the seeds. It turns out that 85% of people don’t really like the seeds in blackberries from our research. And it’s a fairly straightforward path to do that and then remove the thorns as well, so pickers aren’t bleeding.

What impact could CRISPR have on food in a decade?

10 years from now, what I’d like to picture is a lot of produce that not just that has gene editing in it, but is actually more approachable for people. We all grow up being told and taught that we should eat lots of fruits and vegetables, but only 9% of people in the United States eat the recommended amounts of fruits and vegetables. And given 5% are vegetarians, that doesn’t speak real well for the rest of us. So I want to see a variety of things that are more approachable for people.

You mentioned food waste. I think there’s an opportunity to make a substantial difference in shelf life. So when I go into a convenience store, it’s not a choice between a hot dog and a rotten banana. I can get a bowl of berries or something healthy like that as a snack and or pitless cherries. So that’s really our vision is to create a whole different marketplace of foods that fit people’s lifestyles. We eat a lot more food through snacking today than we did 50 years ago and we need to match our food up with that.

To watch the full interview of our interview with Tom Adams or to read the full transcript, just subscribe to Spoon Plus. 

Danone to Use Brightseed’s AI to Uncover New Health Benefits of Soy and Other Plants

Danone North America and Brightseed announced today that they have formed a partnership to use Brightseed’s artificial intelligence (AI) platform to profile and uncover health benefits of key plant sources.

Part of the food as medicine movement, Brightseed is a three-year-old San Francisco startup that examines plants on a molecular level to uncover hidden phytonutrients that can contribute to healthier lifestyles. As it uncovers compounds, Brightseed’s AI platform is then used to predict what impact they will have on the human body.

An example of a phytonutritional compound would be something like the caffeine in coffee or the antioxidants in blueberries.

“We use AI to illuminate the dark matter of nutrition,” Sofia Elizondo, Co-Founder and COO of Brightseed told me by phone this week. “Once you have completed this circle of knowledge. You can transform the food ecosystem.”

Elizondo explained that Brightseed’s platform works for both the sourcing and production sides of CPGs. On the ag side, it can help identify healthy compounds and encourage plant breeding to maximize those benefits. For CPG companies, Brightseed can help source plants that are beneficial and reveal new phytonutrients in existing plant ingredients around which new products can be built.

The partnership with Danone, which owns the Silk and So Delicious Dairy Free brands, will start with Brightseed turning its AI on soy to illuminate the unknown health benefits of soy.

Brightseed, which has raised and undisclosed sum of venture funding, is among a wave of companies using AI to unlock new understandings of our food. Other companies like Spoonshot and Analytical Flavor Systems are using AI to help predict flavor trends and novel food combinations.

But while those companies are looking at existing data, Brightseed is building an entirely new body of data from which entirely new discoveries can be made.

“A lot of technology in our field is built to manipulate nature,” Elizondo said, “There is so much more to learn from what nature has already provided.”

Amid Potato Shortages, People are Worried about French Fries. Could CRISPR Help?

If you’re a french fry lover (guilty), you may have had a mild panic attack this week when news broke that a potato shortage could affect spud supplies. Unusually cold weather in the U.S. and Canada ruined some potato crops, leading to concerns that diminished supply and high demand could lead to a price spike in ‘taters — and a scarcity of french fries.

You don’t need to start stockpiling fries just yet. The New York Times walked back these tater-related fears, noting that consumers “probably shouldn’t worry so much,” and that their french fry suppliers would likely be able to keep up with demand.

This isn’t an isolated incident. With climate change making weather all the more extreme and unpredictable, it’s likely many crops — potatoes and otherwise — will be affected.

One potential solution could be gene editing technologies like CRISPR. Scientists can use these technologies to manipulate the DNA of plants to make them have higher yields and be more resilient to extreme weather conditions. Gene editing is also faster, more accurate, and cheaper than using genetically modified organisms (GMO’s).

If you think that sounds like science fiction… well, it does. But gene editing technology could also be the answer to keeping us in our favorite foods even as populations rise and ocean temperatures rise.

Scientists are already using it to shore up staple crops against the threat of climate change. Yield10, an agriculture bioscience company is developing gene edited seeds for, among other things, potatoes. Companies are also developing new strains of staples like rice and corn that are drought resistant and produce more yield even under harsh weather conditions.

Gene editing can also help non-edible foods become edible; recently scientists figured out how to tweak cotton seeds to make them safe to eat (they’re naturally poisonous).

Similar to GMO’s, gene editing foods, including those made with CRISPR, can be pretty controversial. But as climate change worsens and soil conditions deteriorate, we’ll need to harness a wide variety of agricultural technologies — like gene editing, AI, and maybe even vertical farming — if we want to keep french fries on the menu. That seems reason enough to keep an open mind towards gene editing.

Thanks to Gene Editing, Cotton (Yes, Cotton) Could Be a New Protein Staple

Odds are, you come into contact with cotton every day, in your clothes, your bedsheets, and even your coffee filters.

Soon the “Fabric of Our Lives” might be on your plates, too. Well, sort of. This month the Food and Drug Administration (FDA) declared a genetically engineered form of cotton, developed by Keerti Rathore, a professor of plant biotechnology at Texas A&M University, officially safe to eat. The gene editing only affects the seeds of the plant, not the fluffy stuff used to make your t-shirts, pants, and socks.

Seeds are often used as a source of protein or fat — think sunflower, pumpkin, etc. — but cotton seeds contain gossypol, a chemical that’s harmful to humans. Food companies have already developed a process to remove the chemical from pressed cottonseed oil, which is currently sold in various food products and vegetable oil mixes. However, Rathore’s process, called “RNA interference,” shuts off the gossypol gene in cotton seeds so people can safely eat them in their whole form. It also keeps the gossypol in the rest of the cotton plant, where it acts as a natural pest deterrent.

Since cotton is relatively plentiful, Rathore told Reuters that he hopes that the seeds could be an affordable, nutrient-rich source of protein when added to products like granola, breads or energy bars. Cottonseed meal (what remains after the seeds have been pressed for oil) could also be used to feed fish, pigs and other animals, all of which are also sensitive to gossypol.

However, it’ll be a while before humans (or fish) get a chance to sample these new gene-edited cotton seeds. Rathore hasn’t given an exact timeline, but he told the New Food Economy he first has to license out his technology to seed companies, which can then sell to farmers.

According to Rathore, the impact from adding cotton seeds to the food system could be significant. “There are approximately 10.8 trillion grams of protein locked up in the annual global output of cottonseed,” he told Reuters. “This is enough to meet the basic protein requirements of over 500 million people at a rate of 50 grams of protein per person per day.” Since many of the world’s cotton-producing countries, specifically in African and Asia, struggle with malnutrition, that protein could make a real difference.

The cotton seeds aren’t the first FDA-approved gene-edited food. Earlier this year biotech company Calyxt began selling a gene-edited soybean oil that doesn’t require trans fats for shelf stability. Yield10 Biosciences is developing genetically engineered corn kernels to produce greater outputs. We’ve also been eating FDA-approved genetically modified foods (GMO’s) for decades, especially corn and soy.

Like GMO’s, gene editing foods — including those made using CRISPR — carry a fair bit of controversy. However, the potential benefits are significant. The new gossypol-free cotton seeds give a glimpse into how gene editing can impact our food system. As climate change and deteriorating soil conditions make it harder to cultivate certain crops, gene editing technology could not only save some of our favorite foods — like coffee and chocolate — but could also help us open the door to entirely new protein sources. Throw in a population set to hit roughly 10 billion by 2050, and these sources could be critical to helping us feed a hungry world.

The Food Tech Show: How Will CRISPR Change Food?

Like life, the food we eat is a series of tradeoffs. Whether it’s that banana we buy at the local grocer or that cup of joe we drink at the corner coffee shop, chances are we are not eating or drinking the tastiest or most nutritious variety of the food but instead that which was able to last the longest in transit or is the most disease resistant.

But what if we could have the best of both worlds?

That’s the promise of CRISPR, a new technology that is essentially a form of genetic scissors allows scientists to “edit” DNA gene sequences. Imagine taking out the bad parts of a food’s DNA gene sequence while adding in or changing parts that help make it taste better or last longer.

That’s what scientists are already doing with CRISPR and gene editing techniques.

To discuss this topic, I caught up with the Pete Rowe, the CEO of Deepbranch Biotechnology, for the latest episode of the Food Tech Show podcast.

Rowe, a molecular microbiologist by training, gave the example of avocados as a food that could benefit from CRISPR and gene editing techniques. “Let’s say the best tasting avocados also happen to be the ones that bruise the easiest,” said Rowe. “So you had extremely tasty avocados that you couldn’t ship around the world and therefore they weren’t suited for live scale agriculture. But if you were to make a specific genetic change so that the flavor benefits you get, but you also retain the conventional longevity of this avocado on the shelf.”

But it’s not just helping food last longer, but also helping food survive as a crop.

“Think about all of these problems people are having with coffee,” said Rowe. “Coffee rust is a kinda of fungus that infects coffee plants. There’ s a big genetic component with that. if you make one genetic change within the coffee plant, the likelihood is, if you know what that change has to be, that you can stop that fungus infecting the coffee.

Whether it’s improving flavor, making food more nutritious, or helping it grow faster by speeding up the breeding process, CRISPR and genetic editing hold significant potential.  Rowe does a good job not only explaining these potential applications, but also explains CRISPR in language non scientists like myself (and probably most of our listeners) can understand.

To listen to Rowe talk CRISPR, you can listen to the latest episode of the Food Tech Show on Spotify or Apple Podcasts, download direct to your device, or just click play below.